Computer tomography‐assisted visualization of the movement triggered by frost in Ostrinia nubilalis overwintering in maize stalks

The damage of the European corn borer, Ostrinia nubilalis (Hübner) (Lepidoptera: Crambidae), causes is mainly determined by success its overwintering. aim our study was to assess consequences artificial cooling on movement and survival ability overwintering larvae O. using computer-assisted tomography. in situ examined icy media different thickness (5, 15, 30 cm) during freezing periods 5, 10, 20, 40, 60 min maize stalks CT positioner-laser apparatus. It has been found that ice had a significant effect displacement larvae, however, impact duration moving could not be proven statistically. Enhanced activity due thinner layers 15 were exponential type, which more pronounced just prior point. In contrast, thicker covering (30 caused complete immobility. According results, diapausing still able move albeit it appeared capable surviving direct extreme cold, even leave place as result low temperature. Furthermore, stalk tissue contributes survival-success seems act temperature-buffer moderating severe temperature exerts living tissue.